mirror of
https://github.com/13hannes11/situr.git
synced 2024-09-03 20:50:58 +02:00
282 lines
85 KiB
Plaintext
282 lines
85 KiB
Plaintext
{
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"cells": [
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{
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"cell_type": "code",
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"execution_count": 1,
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"source": [
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"from situr.image import SituImage\n",
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"from situr.registration import PeakFinderDifferenceOfGaussian\n",
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"from situr.registration import FilterregRegistrationFunction, SituImageChannelRegistration\n"
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],
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"outputs": [
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{
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"output_type": "stream",
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"name": "stdout",
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"text": [
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"Jupyter environment detected. Enabling Open3D WebVisualizer.\n",
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"[Open3D INFO] WebRTC GUI backend enabled.\n",
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"[Open3D INFO] WebRTCWindowSystem: HTTP handshake server disabled.\n"
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]
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}
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],
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"metadata": {}
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},
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{
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"cell_type": "code",
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"execution_count": 2,
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"source": [
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"situ_img = SituImage([['./artificial_img/original.tif'], ['./artificial_img/rotated.tif']])\n",
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"situ_img.get_data()"
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],
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"outputs": [
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{
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"output_type": "execute_result",
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"data": {
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"text/plain": [
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"array([[[[0, 0, 0, ..., 0, 0, 0],\n",
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" [0, 0, 0, ..., 0, 0, 0],\n",
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" [0, 0, 0, ..., 0, 0, 0],\n",
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" ...,\n",
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" [0, 0, 0, ..., 0, 0, 0],\n",
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" [0, 0, 0, ..., 0, 0, 0],\n",
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" [0, 0, 0, ..., 0, 0, 0]]],\n",
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"\n",
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"\n",
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" [[[0, 0, 0, ..., 0, 0, 0],\n",
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" [0, 0, 0, ..., 0, 0, 0],\n",
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" [0, 0, 0, ..., 0, 0, 0],\n",
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" ...,\n",
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" [0, 0, 0, ..., 0, 0, 0],\n",
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" [0, 0, 0, ..., 0, 0, 0],\n",
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" [0, 0, 0, ..., 0, 0, 0]]]], dtype=uint16)"
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]
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},
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"metadata": {},
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"execution_count": 2
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}
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],
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"metadata": {}
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},
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{
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"cell_type": "code",
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"execution_count": 3,
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"source": [
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"peak_finder = PeakFinderDifferenceOfGaussian(threshold=0.01)\n",
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"peaks = peak_finder.get_channel_peaks(situ_img, 0)\n",
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"peaks.shape"
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],
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"outputs": [
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{
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"output_type": "execute_result",
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"data": {
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"text/plain": [
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"(8, 2)"
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]
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},
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"metadata": {},
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"execution_count": 3
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}
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],
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"metadata": {}
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},
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{
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"cell_type": "code",
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"execution_count": null,
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"source": [],
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"outputs": [],
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"metadata": {}
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},
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{
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"cell_type": "code",
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"execution_count": 4,
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"source": [
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"peak_finder.get_channel_peaks(situ_img, 0)"
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],
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"outputs": [
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{
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"output_type": "execute_result",
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"data": {
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"text/plain": [
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"array([[ 355., 896.],\n",
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" [ 398., 1490.],\n",
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" [ 818., 1061.],\n",
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" [1481., 872.],\n",
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" [1442., 341.],\n",
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" [ 965., 338.],\n",
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" [ 318., 305.],\n",
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" [1419., 1644.]])"
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]
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},
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"metadata": {},
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"execution_count": 4
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}
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],
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"metadata": {}
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},
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{
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"cell_type": "code",
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"execution_count": 5,
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"source": [
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"peak_finder.show_channel_peaks(situ_img, 0, img_show=False)"
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],
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"outputs": [
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{
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"output_type": "execute_result",
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"data": {
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"text/plain": [
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"<PIL.Image.Image image mode=RGB size=2048x2048 at 0x7F6D3EAFCD30>"
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],
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},
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"metadata": {},
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"execution_count": 5
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}
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],
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"metadata": {}
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},
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{
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"cell_type": "code",
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"execution_count": 6,
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"source": [
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"peak_finder.show_channel_peaks(situ_img, 1, img_show=False)"
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],
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"outputs": [
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{
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"output_type": "execute_result",
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"data": {
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"text/plain": [
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],
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|
|
},
|
|
"metadata": {},
|
|
"execution_count": 6
|
|
}
|
|
],
|
|
"metadata": {}
|
|
},
|
|
{
|
|
"cell_type": "code",
|
|
"execution_count": 7,
|
|
"source": [
|
|
"registration = SituImageChannelRegistration(FilterregRegistrationFunction(), peak_finder=peak_finder)\n",
|
|
"registration.do_channel_registration(situ_img)\n",
|
|
"\n",
|
|
"print(situ_img.get_channel(0))\n",
|
|
"situ_img.apply_transformations()\n",
|
|
"print(situ_img.get_channel(1))"
|
|
],
|
|
"outputs": [
|
|
{
|
|
"output_type": "stream",
|
|
"name": "stdout",
|
|
"text": [
|
|
"[[[0 0 0 ... 0 0 0]\n",
|
|
" [0 0 0 ... 0 0 0]\n",
|
|
" [0 0 0 ... 0 0 0]\n",
|
|
" ...\n",
|
|
" [0 0 0 ... 0 0 0]\n",
|
|
" [0 0 0 ... 0 0 0]\n",
|
|
" [0 0 0 ... 0 0 0]]]\n",
|
|
"[[[0 0 0 ... 0 0 0]\n",
|
|
" [0 0 0 ... 0 0 0]\n",
|
|
" [0 0 0 ... 0 0 0]\n",
|
|
" ...\n",
|
|
" [0 0 0 ... 0 0 0]\n",
|
|
" [0 0 0 ... 0 0 0]\n",
|
|
" [0 0 0 ... 0 0 0]]]\n"
|
|
]
|
|
}
|
|
],
|
|
"metadata": {}
|
|
},
|
|
{
|
|
"cell_type": "code",
|
|
"execution_count": 8,
|
|
"source": [
|
|
"peak_finder.show_channel_peaks(situ_img, 0, img_show=False)"
|
|
],
|
|
"outputs": [
|
|
{
|
|
"output_type": "execute_result",
|
|
"data": {
|
|
"text/plain": [
|
|
"<PIL.Image.Image image mode=RGB size=2048x2048 at 0x7F6D3EB09EB0>"
|
|
],
|
|
"image/png": 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|
|
},
|
|
"metadata": {},
|
|
"execution_count": 8
|
|
}
|
|
],
|
|
"metadata": {}
|
|
},
|
|
{
|
|
"cell_type": "code",
|
|
"execution_count": 9,
|
|
"source": [
|
|
"peak_finder.show_channel_peaks(situ_img, 1, img_show=False)"
|
|
],
|
|
"outputs": [
|
|
{
|
|
"output_type": "execute_result",
|
|
"data": {
|
|
"text/plain": [
|
|
"<PIL.Image.Image image mode=RGB size=2048x2048 at 0x7F6D3EB09A90>"
|
|
],
|
|
"image/png": 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|
|
},
|
|
"metadata": {},
|
|
"execution_count": 9
|
|
}
|
|
],
|
|
"metadata": {}
|
|
},
|
|
{
|
|
"cell_type": "code",
|
|
"execution_count": 10,
|
|
"source": [
|
|
"print(situ_img.channel_transformations[1].offset)\n",
|
|
"print(situ_img.channel_transformations[1].scale)\n",
|
|
"print(situ_img.channel_transformations[1].transform_matrix)"
|
|
],
|
|
"outputs": [
|
|
{
|
|
"output_type": "stream",
|
|
"name": "stdout",
|
|
"text": [
|
|
"[-192.46771011 4.13591988]\n",
|
|
"1.0\n",
|
|
"[[ 1.00000026e+00 -1.65759981e-04]\n",
|
|
" [ 1.65759981e-04 1.00000026e+00]]\n"
|
|
]
|
|
}
|
|
],
|
|
"metadata": {}
|
|
}
|
|
],
|
|
"metadata": {
|
|
"orig_nbformat": 4,
|
|
"language_info": {
|
|
"name": "python",
|
|
"version": "3.8.10",
|
|
"mimetype": "text/x-python",
|
|
"codemirror_mode": {
|
|
"name": "ipython",
|
|
"version": 3
|
|
},
|
|
"pygments_lexer": "ipython3",
|
|
"nbconvert_exporter": "python",
|
|
"file_extension": ".py"
|
|
},
|
|
"kernelspec": {
|
|
"name": "python3",
|
|
"display_name": "Python 3.8.10 64-bit ('env_situr': conda)"
|
|
},
|
|
"interpreter": {
|
|
"hash": "5e38d59b60259c6d967afb47ee3df0fd55767bf61f38f6c5673d6e4df5d1bec7"
|
|
}
|
|
},
|
|
"nbformat": 4,
|
|
"nbformat_minor": 2
|
|
} |