From f17aa2153b12827186d9a299a29f4548ac433fd7 Mon Sep 17 00:00:00 2001 From: "Hannes F. Kuchelmeister" Date: Wed, 21 Jul 2021 19:04:45 +0200 Subject: [PATCH] add demo notebook with artificial test images --- notebook/artificial_img/original.tif | Bin 0 -> 335010 bytes notebook/artificial_img/rotated.tif | Bin 0 -> 335180 bytes notebook/image_demo_test.ipynb | 282 +++++++++++++++++++++++++++ notebook/tile_demo_test.ipynb | 123 ++++++++++++ 4 files changed, 405 insertions(+) create mode 100644 notebook/artificial_img/original.tif create mode 100644 notebook/artificial_img/rotated.tif create mode 100644 notebook/image_demo_test.ipynb create mode 100644 notebook/tile_demo_test.ipynb diff --git a/notebook/artificial_img/original.tif b/notebook/artificial_img/original.tif new file mode 100644 index 0000000000000000000000000000000000000000..a24c1c35ef54a6e6d30da0c0f73b4cf49826ac66 GIT binary patch literal 335010 zcmeI*eQX=$0mt#jNz<0JrK4}@pcAe^6Hq(0&u(H{2~}xJo2oBa(v6o*nlAPw^%}om 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situr.image import SituImage\n", + "from situr.registration import PeakFinderDifferenceOfGaussian\n", + "from situr.registration import FilterregRegistrationFunction, SituImageChannelRegistration\n" + ], + "outputs": [ + { + "output_type": "stream", + "name": "stdout", + "text": [ + "Jupyter environment detected. Enabling Open3D WebVisualizer.\n", + "[Open3D INFO] WebRTC GUI backend enabled.\n", + "[Open3D INFO] WebRTCWindowSystem: HTTP handshake server disabled.\n" + ] + } + ], + "metadata": {} + }, + { + "cell_type": "code", + "execution_count": 2, + "source": [ + "situ_img = SituImage([['./artificial_img/original.tif'], ['./artificial_img/rotated.tif']])\n", + "situ_img.get_data()" + ], + "outputs": [ + { + "output_type": "execute_result", + "data": { + "text/plain": [ + "array([[[[0, 0, 0, ..., 0, 0, 0],\n", + " [0, 0, 0, ..., 0, 0, 0],\n", + " [0, 0, 0, ..., 0, 0, 0],\n", + " ...,\n", + " [0, 0, 0, ..., 0, 0, 0],\n", + " [0, 0, 0, ..., 0, 0, 0],\n", + " [0, 0, 0, ..., 0, 0, 0]]],\n", + "\n", + "\n", + " [[[0, 0, 0, ..., 0, 0, 0],\n", + " [0, 0, 0, ..., 0, 0, 0],\n", + " [0, 0, 0, ..., 0, 0, 0],\n", + " ...,\n", + " [0, 0, 0, ..., 0, 0, 0],\n", + " [0, 0, 0, ..., 0, 0, 0],\n", + " [0, 0, 0, ..., 0, 0, 0]]]], dtype=uint16)" + ] + }, + "metadata": {}, + "execution_count": 2 + } + ], + "metadata": {} + }, + { + "cell_type": "code", + "execution_count": 3, + "source": [ + "peak_finder = PeakFinderDifferenceOfGaussian(threshold=0.01)\n", + "peaks = peak_finder.get_channel_peaks(situ_img, 0)\n", + "peaks.shape" + ], + "outputs": [ + { + "output_type": "execute_result", + "data": { + "text/plain": [ + "(8, 2)" + ] + }, + "metadata": {}, + "execution_count": 3 + } + ], + "metadata": {} + }, + { + "cell_type": "code", + "execution_count": null, + "source": [], + "outputs": [], + "metadata": {} + }, + { + "cell_type": "code", + "execution_count": 4, + "source": [ + "peak_finder.get_channel_peaks(situ_img, 0)" + ], + "outputs": [ + { + "output_type": "execute_result", + "data": { + "text/plain": [ + "array([[ 355., 896.],\n", + " [ 398., 1490.],\n", + " [ 818., 1061.],\n", + " [1481., 872.],\n", + " [1442., 341.],\n", + " [ 965., 338.],\n", + " [ 318., 305.],\n", + " [1419., 1644.]])" + ] + }, + "metadata": {}, + "execution_count": 4 + } + ], + "metadata": {} + }, + { + "cell_type": "code", + "execution_count": 5, + "source": [ + "peak_finder.show_channel_peaks(situ_img, 0, img_show=False)" + ], + "outputs": [ + { + "output_type": "execute_result", + "data": { + "text/plain": [ + "" + ], + "image/png": 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+ }, + "metadata": {}, + "execution_count": 6 + } + ], + "metadata": {} + }, + { + "cell_type": "code", + "execution_count": 7, + "source": [ + "registration = SituImageChannelRegistration(FilterregRegistrationFunction(), peak_finder=peak_finder)\n", + "registration.do_channel_registration(situ_img)\n", + "\n", + "print(situ_img.get_channel(0))\n", + "situ_img.apply_transformations()\n", + "print(situ_img.get_channel(1))" + ], + "outputs": [ + { + "output_type": "stream", + "name": "stdout", + "text": [ + "[[[0 0 0 ... 0 0 0]\n", + " [0 0 0 ... 0 0 0]\n", + " [0 0 0 ... 0 0 0]\n", + " ...\n", + " [0 0 0 ... 0 0 0]\n", + " [0 0 0 ... 0 0 0]\n", + " [0 0 0 ... 0 0 0]]]\n", + "[[[0 0 0 ... 0 0 0]\n", + " [0 0 0 ... 0 0 0]\n", + " [0 0 0 ... 0 0 0]\n", + " ...\n", + " [0 0 0 ... 0 0 0]\n", + " [0 0 0 ... 0 0 0]\n", + " [0 0 0 ... 0 0 0]]]\n" + ] + } + ], + "metadata": {} + }, + { + "cell_type": "code", + "execution_count": 8, + "source": [ + "peak_finder.show_channel_peaks(situ_img, 0, img_show=False)" + ], + "outputs": [ + { + "output_type": "execute_result", + "data": { + "text/plain": [ + "" + ], + "image/png": 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+ }, + "metadata": {}, + "execution_count": 9 + } + ], + "metadata": {} + }, + { + "cell_type": "code", + "execution_count": 10, + "source": [ + "print(situ_img.channel_transformations[1].offset)\n", + "print(situ_img.channel_transformations[1].scale)\n", + "print(situ_img.channel_transformations[1].transform_matrix)" + ], + "outputs": [ + { + "output_type": "stream", + "name": "stdout", + "text": [ + "[-192.46771011 4.13591988]\n", + "1.0\n", + "[[ 1.00000026e+00 -1.65759981e-04]\n", + " [ 1.65759981e-04 1.00000026e+00]]\n" + ] + } + ], + "metadata": {} + } + ], + "metadata": { + "orig_nbformat": 4, + "language_info": { + "name": "python", + "version": "3.8.10", + "mimetype": "text/x-python", + "codemirror_mode": { + "name": "ipython", + "version": 3 + }, + "pygments_lexer": "ipython3", + "nbconvert_exporter": "python", + "file_extension": ".py" + }, + "kernelspec": { + "name": "python3", + "display_name": "Python 3.8.10 64-bit ('env_situr': conda)" + }, + "interpreter": { + "hash": "5e38d59b60259c6d967afb47ee3df0fd55767bf61f38f6c5673d6e4df5d1bec7" + } + }, + "nbformat": 4, + "nbformat_minor": 2 +} \ No newline at end of file diff --git a/notebook/tile_demo_test.ipynb b/notebook/tile_demo_test.ipynb new file mode 100644 index 0000000..4966b04 --- /dev/null +++ b/notebook/tile_demo_test.ipynb @@ -0,0 +1,123 @@ +{ + "cells": [ + { + "cell_type": "code", + "execution_count": 4, + "source": [ + "from situr.image import Tile\n", + "from situr.registration import PeakFinderDifferenceOfGaussian\n", + "from situr.registration import CombinedRegistration, AllChannelRoundRegistration, ChannelRegistration\n", + "from PIL import Image\n" + ], + "outputs": [], + "metadata": {} + }, + { + "cell_type": "code", + "execution_count": 5, + "source": [ + "round_list = [[['./artificial_img/original.tif']], [['./artificial_img/rotated.tif']]]" + ], + "outputs": [], + "metadata": {} + }, + { + "cell_type": "code", + "execution_count": 6, + "source": [ + "tile = Tile(round_list)\n", + "tile.to_numpy_array().shape" + ], + "outputs": [ + { + "output_type": "execute_result", + "data": { + "text/plain": [ + "(2, 1, 1, 2048, 2048)" + ] + }, + "metadata": {}, + "execution_count": 6 + } + ], + "metadata": {} + }, + { + "cell_type": "code", + "execution_count": 7, + "source": [ + "peak_finder = PeakFinderDifferenceOfGaussian()\n", + "registration = CombinedRegistration(AllChannelRoundRegistration(peak_finder=peak_finder), ChannelRegistration(peak_finder=peak_finder))\n", + "registration.do_registration_and_transform(tile)" + ], + "outputs": [], + "metadata": {} + }, + { + "cell_type": "code", + "execution_count": 8, + "source": [ + "peak_finder.show_channel_peaks(tile.get_round(0), 0, img_show=False)" + ], + "outputs": [ + { + "output_type": "execute_result", + "data": { + "text/plain": [ + "" + ], + "image/png": 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+ }, + "metadata": {}, + "execution_count": 8 + } + ], + "metadata": {} + }, + { + "cell_type": "code", + "execution_count": 9, + "source": [ + "peak_finder.show_channel_peaks(tile.get_round(1), 0, img_show=False)" + ], + "outputs": [ + { + "output_type": "execute_result", + "data": { + "text/plain": [ + "" + ], + "image/png": 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