diff --git a/notebook/tile_demo_test.ipynb b/notebook/tile_demo_test.ipynb index 41e3412..2dc661e 100644 --- a/notebook/tile_demo_test.ipynb +++ b/notebook/tile_demo_test.ipynb @@ -2,265 +2,266 @@ "cells": [ { "cell_type": "markdown", + "metadata": {}, "source": [ "# Example registration using a single tile\n", "\n", "This notebook shows the example usage of registering a single tile.\n", "\n", "## Imports" - ], - "metadata": {} + ] }, { "cell_type": "code", - "execution_count": 31, + "execution_count": 17, + "metadata": {}, + "outputs": [], "source": [ "from situr.image import Tile\n", "from situr.registration import PeakFinderDifferenceOfGaussian, IcpRegistrationFunction\n", - "from situr.registration import CombinedRegistration, AllChannelRoundRegistration, ChannelRegistration\n", + "from situr.registration import CombinedRegistration, AllChannelRoundRegistration, AcrossRoundChannelRegistration\n", "\n", "from matplotlib import pyplot as plt\n" - ], - "outputs": [], - "metadata": {} + ] }, { "cell_type": "markdown", + "metadata": {}, "source": [ "## Creating the tile" - ], - "metadata": {} + ] }, { "cell_type": "code", - "execution_count": 32, + "execution_count": 18, + "metadata": {}, + "outputs": [], "source": [ "round_list = [\n", " [['./artificial_img/round_0/channel_0.tif'], ['./artificial_img/round_0/channel_1.tif']], \n", " [['./artificial_img/round_1/channel_0.tif'], ['./artificial_img/round_1/channel_1.tif']]\n", "]\n", "tile = Tile(round_list)" - ], - "outputs": [], - "metadata": {} + ] }, { "cell_type": "markdown", + "metadata": {}, "source": [ "## Convert to numpy array if desired\n", "\n", "The tile can be converted to a numpy array if you so desire.\n" - ], - "metadata": {} + ] }, { "cell_type": "code", - "execution_count": 33, - "source": [ - "tile.to_numpy_array().shape" - ], + "execution_count": 19, + "metadata": {}, "outputs": [ { - "output_type": "execute_result", "data": { "text/plain": [ "(2, 2, 1, 2048, 2048)" ] }, + "execution_count": 19, "metadata": {}, - "execution_count": 33 + "output_type": "execute_result" } ], - "metadata": {} + "source": [ + "tile.to_numpy_array().shape" + ] }, { "cell_type": "markdown", + "metadata": {}, "source": [ "## Creating a peak finder" - ], - "metadata": {} + ] }, { "cell_type": "code", - "execution_count": 34, + "execution_count": 20, + "metadata": {}, + "outputs": [], "source": [ "peak_finder = PeakFinderDifferenceOfGaussian()" - ], - "outputs": [], - "metadata": {} + ] }, { "cell_type": "markdown", + "metadata": {}, "source": [ "## Visualizing found peaks\n", "\n", "To visualize the found peaks we get round 0 and visualize channel 0. This allows us to tweak the peak finder if the results are not satisfactory" - ], - "metadata": {} + ] }, { "cell_type": "code", - "execution_count": 35, - "source": [ - "peak_finder.show_channel_peaks(tile.get_round(0), 0, img_show=False)" - ], + "execution_count": 21, + "metadata": {}, "outputs": [ { - "output_type": "execute_result", "data": { + "image/png": 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+ "" + ] }, + "execution_count": 21, "metadata": {}, - "execution_count": 35 + "output_type": "execute_result" } ], - "metadata": {} + "source": [ + "peak_finder.show_channel_peaks(tile.get_round(0), 0, img_show=False)" + ] }, { "cell_type": "markdown", + "metadata": {}, "source": [ "## Performing the registration\n", "\n", "We use a CombinedRegistration to to first register all channels and then register all rounds. The registration here applies the transformations automatically so we should not call it manually afterwards." - ], - "metadata": {} + ] }, { "cell_type": "code", - "execution_count": 36, - "source": [ - "\n", - "registration = CombinedRegistration(\n", - " AllChannelRoundRegistration(registration_function=IcpRegistrationFunction(300),peak_finder=peak_finder), \n", - " ChannelRegistration(registration_function=IcpRegistrationFunction(150), peak_finder=peak_finder))\n", - "registration.do_registration_and_transform(tile)" - ], + "execution_count": 22, + "metadata": {}, "outputs": [], - "metadata": {} + "source": [ + "registration = CombinedRegistration(\n", + " AllChannelRoundRegistration(registration_function=IcpRegistrationFunction(800),peak_finder=peak_finder), \n", + " AcrossRoundChannelRegistration(registration_function=IcpRegistrationFunction(150), peak_finder=peak_finder))\n", + "registration.do_registration_and_transform(tile)" + ] }, { "cell_type": "markdown", + "metadata": {}, "source": [ "### Visualizing the result\n", "\n", "We show the reference round with it's reference channel, one of its other channels and the modified round to get an idea of the resulting transformaton and how well they are alligned. Also we overlay the peaks in different colours in a scatterplot.\n" - ], - "metadata": {} + ] }, { "cell_type": "code", - "execution_count": 37, + "execution_count": 23, + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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- }, - "metadata": {}, - "execution_count": 39 - } - ], - "metadata": {} + ] }, { "cell_type": "code", - "execution_count": 40, - "source": [ - "peak_finder.scatterplot_channel_peaks(tile.get_round(0), 0, color='r')\n", - "peak_finder.scatterplot_channel_peaks(tile.get_round(1), 0, color='g')\n", - "peak_finder.scatterplot_channel_peaks(tile.get_round(0), 1, color='b')\n", - "plt.show()" - ], + "execution_count": 26, + "metadata": {}, "outputs": [ { - "output_type": "display_data", "data": { + "image/png": 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" + ] }, "metadata": { "needs_background": "light" - } + }, + "output_type": "display_data" } ], - "metadata": {} + "source": [ + "peak_finder.scatterplot_channel_peaks(tile.get_round(0), 1, color='r')\n", + "peak_finder.scatterplot_channel_peaks(tile.get_round(1), 0, color='g')\n", + "peak_finder.scatterplot_channel_peaks(tile.get_round(0), 0, color='b')\n", + "plt.legend(['Round 0 Channel 1', 'Round 1 Channel 0', 'Round 0 Channel 0'])\n", + "plt.show()" + ] } ], "metadata": { - "orig_nbformat": 4, + "interpreter": { + "hash": "916dbcbb3f70747c44a77c7bcd40155683ae19c65e1c03b4aa3499c5328201f1" + }, + "kernelspec": { + "display_name": "Python 3.8.10 64-bit", + "name": "python3" + }, "language_info": { - "name": "python", - "version": "3.8.10", - "mimetype": "text/x-python", "codemirror_mode": { "name": "ipython", "version": 3 }, - "pygments_lexer": "ipython3", + "file_extension": ".py", + "mimetype": "text/x-python", + "name": "python", "nbconvert_exporter": "python", - "file_extension": ".py" + "pygments_lexer": "ipython3", + "version": "3.8.10" }, - "kernelspec": { - "name": "python3", - "display_name": "Python 3.8.10 64-bit ('env_situr': conda)" - }, - "interpreter": { - "hash": "5e38d59b60259c6d967afb47ee3df0fd55767bf61f38f6c5673d6e4df5d1bec7" - } + "orig_nbformat": 4 }, "nbformat": 4, "nbformat_minor": 2 -} \ No newline at end of file +} diff --git a/situr/registration/channel_registration.py b/situr/registration/channel_registration.py index 884db39..65f5e63 100644 --- a/situr/registration/channel_registration.py +++ b/situr/registration/channel_registration.py @@ -45,7 +45,8 @@ class ChannelRegistration(Registration): reference_channel (int, optional): the reference channel that all channels are registered against. Defaults to 0. """ - registration = SituImageChannelRegistration() + + registration = SituImageChannelRegistration(self.registration_function, peak_finder=self.peak_finder) # For each channel (except nucleus) compute transform compared to reference_channel # Add Channel transformation to Channel for round in range(tile.get_round_count()):